Adapter
Sequencing adapter测序接头建库时人工连接到样本片段上的技术序列。
A synthetic oligonucleotide enabling amplification, indexing and sequencing of library fragments.
ATLAS LOOKUP
Glossary
术语表
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Alignment
Sequence alignment序列比对计算一条 read 与参考序列中候选位置的对应关系。
The computational assignment of sequence reads to candidate reference positions under an explicit scoring model.
Doublet
Cell doublet双细胞液滴一个液滴误装入两个或更多细胞。
A barcode containing transcripts from multiple captured cells, potentially creating hybrid profiles.
FASTQ
FASTQ format原始序列与质量文件保存每条测序 read 的碱基和质量。
A text format storing sequence reads and per-base quality scores.
HVG
Highly Variable Genes高变基因优先选择能区分细胞状态的变化基因。
Features selected after modeling expression-dependent variability for downstream representation learning.
Marker gene
Marker gene标志基因在某个细胞群中具有特征表达的基因。
A gene showing enriched expression in a target population relative to a defined comparison.
PCA
Principal Component Analysis主成分分析把数千个基因压缩成少量主要变化轴。
A linear dimensionality-reduction method producing orthogonal components of major variation.
Phred score
Phred quality score碱基质量分数用对数尺度表示碱基调用错误概率的模型估计。
A logarithmic score Q = -10 log10(Perror) encoding an estimated base-calling error probability.
Read
Sequencing read测序读段测序仪对一个文库片段进行的有限长度序列观察。
A base-called sequence observation derived from one end of a library fragment, accompanied by per-base quality estimates.
resolution
Clustering resolution聚类粒度控制邻居图被划分得更粗还是更细。
A graph-clustering granularity parameter whose interpretation depends on stability and biological evidence.
UMAP
Uniform Manifold Approximation and Projection统一流形逼近与投影把高维细胞关系投影到二维，便于观察。
A nonlinear embedding that visualizes local neighborhood structure without providing a statistical test.
UMI
Unique Molecular Identifier唯一分子标识符给原始 RNA 分子贴上的短标签，用来识别后续产生的 PCR 拷贝。
A short random sequence used to distinguish original captured molecules from amplification duplicates.